Publications

Publications

2021

A circadian clock in the sinus node mediates day-night rhythms in Hcn4 and heart rate.

Heart Rhythm 2021 May;18(5):801-810. doi:10.1016/j.hrthm.2020.11.026. Epub 2020 Dec 3. PMID: 33278629; PMCID: PMC8073545
D'Souza A, Wang Y, Anderson C, Bucchi A, Baruscotti M, Olieslagers S, Mesirca P, Johnsen AB, Mastitskaya S, Ni H, Zhang Y, Black N, Cox C, Wegner S, Bano-Otalora B, Petit C, Gill E, Logantha SJRJ, Dobrzynski H, Ashton N, Hart G, Zhang R, Zhang H, Cartwright EJ, Wisloff U, Mangoni ME, da Costa Martins PA, Piggins HD, DiFrancesco D, Boyett MR. (2021)

Age-related changes in cardiac autonomic modulation and heart rate variability in mice.

Frontiers in Neuroscience 15:617698. doi: 10.3389/fnins.2021.617698
Piantoni C, Carnevali L, Molla D, Barbuti A, DiFrancesco D, Bucchi A, Baruscotti M (2021)

Age-related changes in cardiac autonomic modulation and heart rate variability in mice.

Frontiers in Neuroscience (in the press)
Piantoni C, Baruscotti M, Carnevali L, Molla D, Barbuti A, DiFrancesco D, Bucchi A (2021)

2019

HCN ion channels and accessory proteins in epilepsy: genetic analysis of a large cohort of patients and review of the literature

Epilepsy Res. 2019 Jul;153:49-58. doi: 10.1016/j.eplepsyres.2019.04.004.
DiFrancesco JC, Castellotti B, Milanesi R, Ragona F, Freri E, Canafoglia L, Franceschetti S, Ferrarese C, Magri S, Taroni F, Costa C, Labate A, Gambardella A, Solazzi R, Binda A, Rivolta I, Di Gennaro G, Casciato S, D'Incerti L, Barbuti A, DiFrancesco D, Granata T, Gellera C.

Human iPSC modeling of a familial form of atrial fibrillation reveals a gain of function of If and ICaL in patient-derived cardiomyocytes.

Cardiovascular Research doi: 10.1093/cvr/cvz217
Benzoni P, Campostrini G, Landi S, Bertini V, Marchina E, Iascone M, Ahlberg G, Olesen MS, Crescini E, Mora C, Bisleri G, Muneretto C, Ronca R, Presta M, Poliani PL, Piovani G, Verardi R, Pasquale ED, Consiglio A, Raya A, Torre E, Lodrini AM, Milanesi R, Rocchetti M, Baruscotti M, DiFrancesco D, Memo M, Barbuti A, Dell'Era P. (2019)

Comparing pathways for long-term heart rate modulation by the funny current.

Journal of General Physiology 151(9):1066-1069
DiFrancesco JC, Castellotti B, Milanesi R, Ragona F, Freri E, Canafoglia L, Franceschetti S, Ferrarese C, Magri S, Taroni F, Costa C, Labate A, Gambardella A, Solazzi R, Binda A, Rivolta I, Di Gennaro G, Casciato S, D'Incerti L, Barbuti A, DiFrancesco D, Granata T, Gellera C.  (2019)

2018

Screening and Identification of Cardioprotective Compounds From Wenxin Keli by Activity Index Approach and in vivo Zebrafish Model.

Front Pharmacol. 2018 Nov 13;9:1288. doi: 10.3389/fphar.2018.01288. eCollection 2018.
Liu H, Chen X, Zhao X, Zhao B, Qian K, Shi Y, Baruscotti M, Wang Y.

HCN1 mutation spectrum: from neonatal epileptic encephalopathy to benign generalized epilepsy and beyond

Brain. 2018 Nov 1;141(11):3160-3178. doi: 10.1093/brain/awy263.
Marini C, Porro A, Rastetter A, Dalle C, Rivolta I, Bauer D, Oegema R, Nava C, Parrini E, Mei D, Mercer C, Dhamija R, Chambers C, Coubes C, Thévenon J, Kuentz P, Julia S, Pasquier L, Dubourg C, Carré W, Rosati A, Melani F, Pisano T, Giardino M, Innes AM, Alembik Y, Scheidecker S, Santos M, Figueiroa S, Garrido C, Fusco C, Frattini D, Spagnoli C, Binda A, Granata T, Ragona F, Freri E, Franceschetti S, Canafoglia L, Castellotti B, Gellera C, Milanesi R, Mancardi MM, Clark DR, Kok F, Helbig KL, Ichikawa S, Sadler L, Neupauerová J, Laššuthova P, Šterbová K, Laridon A, Brilstra E, Koeleman B, Lemke JR, Zara F, Striano P, Soblet J, Smits G, Deconinck N, Barbuti A, DiFrancesco D, LeGuern E, Guerrini R, Santoro B, Hamacher K, Thiel G, Moroni A, DiFrancesco JC, Depienne C.

A novel de novo HCN1 loss-of-function mutation in genetic generalized epilepsy causing increased neuronal excitability

Neurobiol Dis. 2018 Oct;118:55-63. doi: 10.1016/j.nbd.2018.06.012.
Bonzanni M, DiFrancesco JC, Milanesi R, Campostrini G, Castellotti B, Bucchi A, Baruscotti M, Ferrarese C, Franceschetti S, Canafoglia L, Ragona F, Freri E, Labate A, Gambardella A, Costa C, Rivolta I, Gellera C, Granata T, Barbuti A, DiFrancesco D.

A Loss-of-Function HCN4 Mutation Associated With Familial Benign Myoclonic Epilepsy in Infancy Causes Increased Neuronal Excitability.

Front Mol Neurosci. 2018 Aug 6;11:269. doi: 10.3389/fnmol.2018.00269. eCollection 2018.
Campostrini G, DiFrancesco JC, Castellotti B, Milanesi R, Gnecchi-Ruscone T, Bonzanni M, Bucchi A, Baruscotti M, Ferrarese C, Franceschetti S, Canafoglia L, Ragona F, Freri E, Labate A, Gambardella A, Costa C, Gellera C, Granata T, Barbuti A, DiFrancesco D.

Modulation of the intrinsic neuronal excitability by multifunctional liposomes tailored for the treatment of Alzheimer’s disease.

Int J Nanomedicine. 2018 Jul 11;13:4059-4071. doi: 10.2147/IJN.S161563. eCollection 2018.
Binda A, Panariti A, Barbuti A, Murano C, Dal Magro R, Masserini M, Re F, Rivolta I.

A synthetic peptide that prevents cAMP regulation in mammalian Hyperpolarization-activated Cyclic Nucleotide-regulated (HCN) channels.

Elife. 2018 Jun 20;7. pii: e35753. doi: 10.7554/eLife.35753.
Saponaro A, Cantini F, Porro A, Bucchi A, DiFrancesco D, Maione V, Donadoni C, Introini B, Mesirca P, Mangoni ME, Thiel G, Banci L, Santoro B, Moroni A.

Effects of repeated sprints training on fracture risk-associated miRNA

Oncotarget. 2018 Apr 6;9(26):18029-18040. doi: 10.18632/oncotarget.24707.
Sansoni V, Perego S, Vernillo G, Barbuti A, Merati G, La Torre A, Banfi G, Lombardi G.

Generation of human induced pluripotent stem cells (EURACi001-A, EURACi002-A, EURACi003-A) from peripheral blood mononuclear cells of three patients carrying mutations in the CAV3 gene.

Stem Cell Research
Volume 27, March 2018, Pages 25-29.
doi: 10.1016/j.scr.2017.12.012.
Meraviglia V, Benzoni P, Landi S, Murano C, Langione M, Motta BM, Baratto S, Silipigni R, Di Segni M, Pramstaller PP, DiFrancesco D, Gazzerro E, Barbuti A, Rossini A.

Preferential myofibroblast differentiation of cardiac mesenchymal progenitor cells in the presence of atrial fibrillation.

Transl Res. 2018 Feb;192:54-67. doi: 10.1016/j.trsl.2017.11.003
Gambini E, Perrucci GL, Bassetti B, Spaltro G, Campostrini G, Lionetti MC, Pilozzi A, Martinelli F, Faruggia A, DiFrancesco D, Barbuti A, Pompilio G.

2017

Structure-guided design of a cell penetrating peptide preventing cAMP modulation of HCN channels

bioRxiv, 253096 doi: https://doi.org/10.1101/253096
This article is a preprint and has not been peer-reviewed
Anna Moroni, Andrea Saponaro, Francesca Cantini, Alessandro Porro, Annalisa Bucchi, Dario DiFrancesco, Vincenzo Maione, Chiara Donadoni, Bianca Introini, Pietro Mesirca, Matteo E. Mangoni, Gerhard Thiel, Lucia Banci, Bina Santoro.

Mammalian γ2 AMPK regulates intrinsic heart rate.

Nat Commun. 2017 Nov 2;8(1):1258. doi: 10.1038/s41467-017-01342-5.
Yavari A, Bellahcene M, Bucchi A, Sirenko S, Pinter K, Herring N, Jung JJ, Tarasov KV, Sharpe EJ, Wolfien M, Czibik G, Steeples V, Ghaffari S, Nguyen C, Stockenhuber A, Clair JRS, Rimmbach C, Okamoto Y, Yang D, Wang M, Ziman BD, Moen JM, Riordon DR, Ramirez C, Paina M, Lee J, Zhang J, Ahmet I, Matt MG, Tarasova YS, Baban D, Sahgal N, Lockstone H, Puliyadi R, de Bono J, Siggs OM, Gomes J, Muskett H, Maguire ML, Beglov Y, Kelly M, Dos Santos PPN, Bright NJ, Woods A, Gehmlich K, Isackson H, Douglas G, Ferguson DJP, Schneider JE, Tinker A, Wolkenhauer O, Channon KM, Cornall RJ, Sternick EB, Paterson DJ, Redwood CS, Carling D, Proenza C, David R, Baruscotti M, DiFrancesco D, Lakatta EG, Watkins H, Ashrafian H.

The expression of the rare caveolin-3 variant T78M alters cardiac ion channels function and membrane excitability.

Cardiovasc Res. 2017 Aug 1;113(10):1256-1265. doi: 10.1093/cvr/cvx122.
Campostrini G, Bonzanni M, Lissoni A, Bazzini C, Milanesi R, Vezzoli E, Francolini M, Baruscotti M, Bucchi A, Rivolta I, Fantini M, Severi S, Cappato R, Crotti L, J Schwartz P, DiFrancesco D, Barbuti A.

Bone turnover response is linked to both acute and established metabolic changes in ultra-marathon runners.

Endocrine. 2017 Apr;56(1):196-204. doi: 10.1007/s12020-016-1012-8.
Sansoni V, Vernillo G, Perego S, Barbuti A, Merati G, Schena F, La Torre A, Banfi G, Lombardi G.

A Traditional Chinese Medicine Drug (TMYX) Controls Heart Rate by Modulation of the Pacemaker (F) Channels

Biophysical Journal, Volume 112, Issue 3, Supplement 1, p412a, 3 February 2017. DOI: 10.1016/j.bpj.2016.11.2565
Manuel Paina, Chiara Piantoni, Luca Carnevali, Anthony Frosio, Annalisa Bucchi, Andrea Barbuti, Liu Sheng, Wang Yanyan, Yi Wang, Dario DiFrancesco, Mirko Baruscotti

HCN3 Channel Expression in Human Leukocytes

Biophysical Journal, Volume 112, Issue 3, Supplement 1, p415a, 3 February 2017. doi: 10.1016/j.bpj.2016.11.2224
Chiara Piantoni, Angelica Gualdoni, Claudia Bazzini, Raffaella Milanesi, Miryam Adelfio, Annalisa Bucchi, Andrea Barbuti, Matteo Pecchiari, Mirko Baruscotti, Dario DiFrancesco

A gain-of-function mutation in the cardiac pacemaker HCN4 channel increasing cAMP sensitivity is associated with familial Inappropriate Sinus Tachycardia.

Eur Heart J. 2017 Jan 21;38(4):280-288. doi: 10.1093/eurheartj/ehv582.
Baruscotti M, Bucchi A, Milanesi R, Paina M, Barbuti A, Gnecchi-Ruscone T, Bianco E, Vitali-Serdoz L, Cappato R, DiFrancesco D.

Plasminogen activator inhibitor-1 as a marker of cardiovascular response in professional mountain ultra-marathon runners.

Clin Chem Lab Med. 2017 Jan 1;55(1):e7-e9. doi: 10.1515/cclm-2016-0295.
Lombardi G, Vernillo G, Sansoni V, Perego S, Barbuti A, Merati G, Schena F, Banfi G, La Torre A.

2016

Human derived cardiomyocytes: A decade of knowledge after the discovery of induced pluripotent stem cells

Dev Dyn. 2016 Dec;245(12):1145-1158. doi: 10.1002/dvdy.24455.
Barbuti A, Benzoni P, Campostrini G, Dell'Era P.

Anacardic acid and thyroid hormone enhance cardiomyocytes production from undifferentiated mouse ES cells along functionally distinct pathways.

Endocrine. 2016 Sep;53(3):681-8. doi: 10.1007/s12020-015-0751-2. Epub 2015 Nov 7.
Re A, Nanni S, Aiello A, Granata S, Colussi C, Campostrini G, Spallotta F, Mattiussi S, Pantisano V, D'Angelo C, Biroccio A, Rossini A, Barbuti A, DiFrancesco D, Trimarchi F, Pontecorvi A, Gaetano C, Farsetti A.

Mutation in S6 domain of HCN4 channel in patient with suspected Brugada syndrome modifies channel function.

Pflugers Arch. 2016 Oct;468(10):1663-71. doi: 10.1007/s00424-016-1870-1. Epub 2016 Aug 23.
Biel S, Aquila M, Hertel B, Berthold A, Neumann T, DiFrancesco D, Moroni A, Thiel G, Kauferstein S.

Current understanding of the pathophysiological mechanisms responsible for inappropriate sinus tachycardia: role of the If “funny” current.

J Interv Card Electrophysiol. 2016 Jun;46(1):19-28. doi: 10.1007/s10840-015-0097-y. Epub 2016 Jan 18.
Baruscotti M, Bianco E, Bucchi A, DiFrancesco D.

INaP selective inhibition reverts precocious inter- and motorneurons hyperexcitability in the Sod1-G93R zebrafish ALS model.

Sci Rep. 2016 Apr 15;6:24515. doi: 10.1038/srep24515.
Benedetti L, Ghilardi A, Rottoli E, De Maglie M, Prosperi L, Perego C, Baruscotti M, Bucchi A, Del Giacco L, Francolini M.

Higher cardiogenic potential of iPSCs derived from cardiac versus skin stromal cells.

Front Biosci (Landmark Ed). 2016 Jan 1;21:719-43.
Meraviglia V, Wen J, Piacentini L, Campostrini G, Wang C, Florio MC, Azzimato V, Fassina L, Langes M, Wong J, Miragoli M, Gaetano C, Pompilio G, Barbuti A, DiFrancesco D, Mascalzoni D, Pramstaller PP, Colombo GI, Chen HS, Rossini A.

Cell-specific Dynamic Clamp analysis of the role of funny If current in cardiac pacemaking.

Prog Biophys Mol Biol. 2016 Jan;120(1-3):50-66. doi: 10.1016/j.pbiomolbio.2015.12.004. Epub 2015 Dec 21.
Ravagli E, Bucchi A, Bartolucci C, Paina M, Baruscotti M, DiFrancesco D, Severi S.

2015

Postexercise autonomic function after repeated-sprints training

Eur J Appl Physiol. 2015 Nov;115(11):2445-55. doi: 10.1007/s00421-015-3226-5.
Vernillo G, Agnello L, Barbuti A, Di Meco S, Lombardi G, Merati G, La Torre A.

Acetylation mediates Cx43 reduction caused by electrical stimulation.

J Mol Cell Cardiol. 2015 Oct;87:54-64. doi: 10.1016/j.yjmcc.2015.08.001.
Meraviglia V, Azzimato V, Colussi C, Florio MC, Binda A, Panariti A, Qanud K, Suffredini S, Gennaccaro L, Miragoli M, Barbuti A, Lampe PD, Gaetano C, Pramstaller PP, Capogrossi MC, Recchia FA, Pompilio G, Rivolta I, Rossini A.

The genetic basis for inherited forms of sinoatrial dysfunction and atrioventricular node dysfunction.

J Interv Card Electrophysiol. 2015 Aug;43(2):121-34. doi: 10.1007/s10840-015-9998-z.
Milanesi R, Bucchi A, Baruscotti M.

Stem cell-derived nodal-like cardiomyocytes as a novel pharmacologic tool: insights from sinoatrial node development and function.

Pharmacol Rev. 2015;67(2):368-88. doi: 10.1124/pr.114.009597.
Barbuti A, Robinson RB.

HCN4, Sinus Bradycardia and Atrial Fibrillation.

Arrhythm Electrophysiol Rev. 2015 May;4(1):9-13. doi: 10.15420/aer.2015.4.1.9. Epub 2015 Mar 15.
DiFrancesco D.

Dysfunctional HCN ion channels in neurological diseases.

Front Cell Neurosci. 2015 Mar 10;6:174. doi: 10.3389/fncel.2015.00071. eCollection 2015.
DiFrancesco JC, DiFrancesco D.

2014

LEONARDO DA VINCI AND THE ORIGIN OF SEMEN.

Notes Rec R Soc Lond. 2014 Dec 20;68(4):391-402.
Noble D, DiFrancesco D, Zancani D.

Cyclic dinucleotides bind the C-linker of HCN4 to control channel cAMP responsiveness.

Nat Chem Biol. 2014 Jun;10(6):457-62.
Lolicato M, Bucchi A, Arrigoni C, Zucca S, Nardini M, Schroeder I, Simmons K, Aquila M, DiFrancesco D, Bolognesi M, Schwede F, Kashin D, Fishwick CW, Johnson AP, Thiel G, Moroni A.

Exercise training reduces resting heart rate via downregulation of the funny channel HCN4.

Nat Commun. 2014 May 13;5:3775.
D'Souza A, Bucchi A, Johnsen AB, Logantha SJ, Monfredi O, Yanni J, Prehar S, Hart G, Cartwright E, Wisloff U, Dobryznski H, DiFrancesco D, Morris GM, Boyett MR.

The ‘funny’ side of sepsis.

J Physiol. 2014 Mar 15;592(6):1171.
Barbuti A, DiFrancesco D.

2013

Multifactorial impact of channel beta-subunit gene mutation on automaticity.

J Cardiovasc Electrophysiol. 2013 Dec;24(12):E26-7.
Difrancesco D, Nawathe PA, Robinson RB.

Funny channel gene mutations associated with arrhythmias.

J Physiol. 2013 Sep 1;591(17):4117-24.
DiFrancesco D.

An LQTS6 MiRP1 mutation suppresses pacemaker current and is associated with sinus bradycardia.

J Cardiovasc Electrophysiol. 2013 Sep;24(9):1021-7.
Nawathe PA, Kryukova Y, Oren RV, Milanesi R, Clancy CE, Lu JT, Moss AJ, Difrancesco D, Robinson RB.

Embryonic stem cell-derived CD166+ precursors develop into fully functional sinoatrial-like cells.

Circ Res. 2013 Aug 2;113(4):389-98.
DiFrancesco D, Barbuti A.

Identification of the molecular site of ivabradine binding to HCN4 channels.

PLoS One. 2013;8(1):e53132.
Bucchi A, Baruscotti M, Nardini M, Barbuti A, Micheloni S, Bolognesi M, DiFrancesco D.

2012

In vitro epigenetic reprogramming of human cardiac mesenchymal stromal cells into functionally competent cardiovascular precursors.

PLoS One. 2012;7(12):e51694.
Vecellio M, Meraviglia V, Nanni S, Barbuti A, Scavone A, DiFrancesco D, Farsetti A, Pompilio G, Colombo GI, Capogrossi MC, Gaetano C, Rossini A.

Clinical efficacy of ivabradine in patients with inappropriate sinus tachycardia: a prospective, randomized, placebo-controlled, double-blind, crossover evaluation.

J Am Coll Cardiol. 2012 Oct 9;60(15):1323-9.
Cappato R, Castelvecchio S, Ricci C, Bianco E, Vitali-Serdoz L, Gnecchi-Ruscone T, Pittalis M, De Ambroggi L, Baruscotti M, Gaeta M, Furlanello F, Di Francesco D, Lupo PP.

An updated computational model of rabbit sinoatrial action potential to investigate the mechanisms of heart rate modulation.

J Physiol. 2012 Sep 15;590(18):4483-99.
Severi S, Fantini M, Charawi LA, DiFrancesco D.

A caveolin-binding domain in the HCN4 channels mediates functional interaction with caveolin proteins.

J Mol Cell Cardiol. 2012 Aug;53(2):187-95.
Barbuti A, Scavone A, Mazzocchi N, Terragni B, Baruscotti M, Difrancesco D.

Funny Current and Cardiac Rhythm: Insights from HCN Knockout and Transgenic Mouse Models.

Front Physiol. 2012 Jul 2;3:240.
Bucchi A, Barbuti A, Difrancesco D, Baruscotti M.

Rebuttal: “The funny current in the context of the coupled clock pacemaker cell system”

Heart Rhythm. 2012 Mar;9(3):457-8.
DiFrancesco D, Noble D.

2011

Recessive Loss-of-Function Mutation in the Pacemaker HCN2 Channel Causing Increased Neuronal Excitability in a Patient with Idiopathic Generalized Epilepsy

J Neurosci. 2011 Nov 30;31(48):17327-37.
DiFrancesco JC, Barbuti A, Milanesi R, Coco S, Bucchi A, Bottelli G, Ferrarese C, Franceschetti S, Terragni B, Baruscotti M, DiFrancesco D.

Multinucleated giant cells with an osteoclast phenotype derived from caprine peripheral blood mononuclear cells.

Vet J. 2011 Sep;189(3):361-3.
Pisoni G, D'Amelio P, Sassi F, Manarolla G, Scaccabarozzi L, Locatelli C, Mazzocchi N, Baruscotti M, DiFrancesco D, Moroni P.

Human cord blood CD34+ progenitor cells acquire functional cardiac properties through a cell fusion process.

Am J Physiol Heart Circ Physiol. 2011 May;300(5):H1875-84.
Avitabile D, Crespi A, Brioschi C, Parente V, Toietta G, Devanna P, Baruscotti M, Truffa S, Scavone A, Rusconi F, Biondi A, D'Alessandra Y, Vigna E, Difrancesco D, Pesce M, Capogrossi MC, Barbuti A.

Human cardiac and bone marrow stromal cells exhibit distinctive properties related to their origin.

Cardiovasc Res. 2011 Feb 15;89(3):650-60.
Rossini A, Frati C, Lagrasta C, Graiani G, Scopece A, Cavalli S, Musso E, Baccarin M, Di Segni M, Fagnoni F, Germani A, Quaini E, Mayr M, Xu Q, Barbuti A, DiFrancesco D, Pompilio G, Quaini F, Gaetano C, Capogrossi MC.

Deep bradycardia and heart block caused by inducible cardiac-specific knockout of the pacemaker channel gene Hcn4.

Proc Natl Acad Sci USA. 2011 Jan 25;108(4):1705-10.
Baruscotti M, Bucchi A, Viscomi C, Mandelli G, Consalez G, Gnecchi-Rusconi T, Montano N, Casali KR, Micheloni S, Barbuti A, DiFrancesco D.

2010

Mesoangioblasts from ventricular vessels can differentiate in vitro into cardiac myocytes with sinoatrial-like properties.

J Mol Cell Cardiol. 2010 Feb;48(2):415-23.
Barbuti A, Galvez BG, Crespi A, Scavone A, Baruscotti M, Brioschi C, Cossu G, DiFrancesco D.

Funny channel-based pacemaking.

Heart Rhythm. 2010;7(2):276-9
DiFrancesco D.

2009

More seafood to control heart rate?

Heart Rhythm. 2009 Oct;6(10):1493-4.
DiFrancesco D.

Distribution of the pacemaker HCN4 channel mRNA and protein in the rabbit sinoatrial node.

J Mol Cell Cardiol. 2009 Aug;47(2):221-7.
Brioschi C, Micheloni S, Tellez JO, Pisoni G, Longhi R, Moroni P, Billeter R, Barbuti A, Dobrzynski H, Boyett MR, DiFrancesco D, Baruscotti M.

Molecular composition and functional properties of f-channels in murine embryonic stem cell-derived pacemaker cells.

J Mol Cell Cardiol. 2009 Mar;46(3):343-51.
Barbuti A, Crespi A, Capilupo D, Mazzocchi N, Baruscotti M, DiFrancesco D.

2008

Cardiac mesoangioblasts are committed, self-renewable progenitors, associated with small vessels of juvenile mouse ventricle.

Cell Death Differ. 2008 Sep;15(9):1417-28.
Galvez BG, Sampaolesi M, Barbuti A, Crespi A, Covarello D, Brunelli S, Dellavalle A, Crippa S, Balconi G, Cuccovillo I, Molla F, Staszewsky L, Latini R, Difrancesco D, Cossu G.

2007

Modulation of rate by autonomic agonists in SAN cells involves changes in diastolic depolarization and the pacemaker current.

J Mol Cell Cardiol. 2007 Jul;43(1):39-48.
Bucchi A, Baruscotti M, Robinson RB, DiFrancesco D.

Localization of f-channels to caveolae mediates specific beta2-adrenergic receptor modulation of rate in sinoatrial myocytes.

J Mol Cell Cardiol. 2007 Jan;42(1):71-8.
Barbuti A, Terragni B, Brioschi C, DiFrancesco D.

2006

Properties of ivabradine-induced block of HCN1 and HCN4 pacemaker channels.

J Physiol. 2006 Apr 15;572(Pt 2):335-46.
Bucchi A, Tognati A, Milanesi R, Baruscotti M, DiFrancesco D.

Direct inhibition of the pacemaker (If) current in rabbit sinoatrial node cells by genistein.

Br J Pharmacol. 2006 Jan;147(1):36-44.
Altomare C, Tognati A, Bescond J, Ferroni A, Baruscotti M.

Familial sinus bradycardia associated with a mutation in the cardiac pacemaker channel.

N Engl J Med. 2006 Jan 12;354(2):151-7.
Milanesi R, Baruscotti M, Gnecchi-Ruscone T, DiFrancesco D.

2005

Letter regarding article by Michels et al, “Single-channel properties support a potential contribution of hyperpolarization-activated cyclic nucleotide-gated channels and If to cardiac arrhythmias”.

Circulation. 2005 Aug 2;112(5):e72;
DiFrancesco D.

2004

Interaction of the pacemaker channel HCN1 with filamin A.

J Biol Chem. 2004 Oct 15;279(42):43847-53.
Gravante B, Barbuti A, Milanesi R, Zappi I, Viscomi C, DiFrancesco D.

Long distance interactions within the potassium channel pore are revealed by molecular diversity of viral proteins.

J Biol Chem. 2004 Jul 2;279(27):28443-9.
Gazzarrini S, Kang M, Van Etten JL, Tayefeh S, Kast SM, DiFrancesco D, Thiel G, Moroni A.

Localization of pacemaker channels in lipid rafts regulates channel kinetics.

Circ Res. 2004 May 28;94(10):1325-31.
Barbuti A, Gravante B, Riolfo M, Milanesi R, Terragni B, DiFrancesco D.

Small potassium ion channel proteins encoded by chlorella viruses.

Proc Natl Acad Sci USA. 2004 Apr 13;101(15):5318-24.
Kang M, Moroni A, Gazzarrini S, DiFrancesco D, Thiel G, Severino M, Van Etten JL.

2003

Autonomic modulation of heart rate: pitfalls of nonselective channel blockade.

Am J Physiol Heart Circ Physiol. 2003 Dec;285(6)
Robinson RB, Baruscotti M, DiFrancesco D.

I(f)-dependent modulation of pacemaker rate mediated by cAMP in the presence of ryanodine in rabbit sino-atrial node cells.

J Mol Cell Cardiol. 2003 Aug;35(8):905-13.
Bucchi A, Baruscotti M, Robinson RB, DiFrancesco D.

Heteromeric HCN1-HCN4 channels: a comparison with native pacemaker channels from the rabbit sinoatrial node.

J Physiol. 2003 Jun 1;549(Pt 2):347-59.
Altomare C, Terragni B, Brioschi C, Milanesi R, Pagliuca C, Viscomi C, Moroni A, Baruscotti M, DiFrancesco D.

2002

The short N-terminus is required for functional expression of the virus-encoded miniature K(+) channel Kcv.

FEBS Lett. 2002 Oct 23;530(1-3):65-9.
Moroni A, Viscomi C, Sangiorgio V, Pagliuca C, Meckel T, Horvath F, Gazzarrini S, Valbuzzi P, Van Etten JL, DiFrancesco D, Thiel G.

Functional comparison of HCN isoforms expressed in ventricular and HEK 293 cells.

Pflugers Arch. 2002 Aug;444(5):597-601.
Qu J, Altomare C, Bucchi A, DiFrancesco D, Robinson RB.

Current-dependent block of rabbit sino-atrial node I(f) channels by ivabradine.

J Gen Physiol. 2002 Jul;120(1):1-13.
Bucchi A, Baruscotti M, DiFrancesco D.

Functional characterisation and subcellular localisation of HCN1 channels in rabbit retinal rod photoreceptors.

J Physiol. 2002 Jul 1;542(Pt 1):89-97.
Demontis GC, Moroni A, Gravante B, Altomare C, Longoni B, Cervetto L, DiFrancesco D.

Voltage-dependence of virus-encoded miniature K+ channel Kcv.

J Membr Biol. 2002 May 1;187(1):15-25.
Gazzarrini S, Etten JL, DiFrancesco D, Thiel G, Moroni A.

beta-modulation of pacemaker rate: novel mechanism or novel mechanics of an old one?

Circ Res. 2002 Apr 5;90(6):E69-9.
DiFrancesco D, Robinson RB.

2001

L-type but not T-type calcium current changes during postnatal development in rabbit sinoatrial node.

Am J Physiol Heart Circ Physiol. 2001 Sep;281(3)
Protas L, DiFrancesco D, Robinson RB.

Hyperpolarization-activated cyclic nucleotide-gated channel 1 is a molecular determinant of the cardiac pacemaker current I(f).

J Biol Chem. 2001 Aug 3;276(31):29233-41.
Moroni A, Gorza L, Beltrame M, Gravante B, Vaccari T, Bianchi ME, Altomare C, Longhi R, Heurteaux C, Vitadello M, Malgaroli A, DiFrancesco D.

C terminus-mediated control of voltage and cAMP gating of hyperpolarization-activated cyclic nucleotide-gated channels.

J Biol Chem. 2001 Aug 10;276(32):29930-4.
Viscomi C, Altomare C, Bucchi A, Camatini E, Baruscotti M, Moroni A, DiFrancesco D.

Integrated allosteric model of voltage gating of HCN channels.

J Gen Physiol. 2001 Jun;117(6):519-32.
Altomare C, Bucchi A, Camatini E, Baruscotti M, Viscomi C, Moroni A, DiFrancesco D.

Single-channel properties of the sinoatrial node Na+ current in the newborn rabbit.

Pflugers Arch. 2001 May;442(2):192-6.
Baruscotti M, DiFrancesco D, Robinson RB.

2000

Na(+) current contribution to the diastolic depolarization in newborn rabbit SA node cells.

Am J Physiol Heart Circ Physiol. 2000 Nov;279(5)
Baruscotti M1, DiFrancesco D, Robinson RB.

Effects of dronedarone on acetylcholine-activated current in rabbit SAN cells.

Br J Pharmacol. 2000 Jul;130(6):1315-20.
Altomare C1, Barbuti A, Viscomi C, Baruscotti M, DiFrancesco D.

Mutation in pore domain uncovers cation- and voltage-sensitive recovery from inactivation in KAT1 channel.

Biophys J. 2000 Apr;78(4):1862-71.
Moroni A1, Gazzarrini S, Cerana R, Colombo R, Sutter JU, DiFrancesco D, Gradmann D, Thiel G.

A potassium channel protein encoded by chlorella virus PBCV-1.

Science. 2000 Mar 3;287(5458):1641-4.
Plugge B, Gazzarrini S, Nelson M, Cerana R, Van Etten JL, Derst C, DiFrancesco D, Moroni A, Thiel G.

Kinetic and ionic properties of the human HCN2 pacemaker channel.

Pflugers Arch. 2000 Mar;439(5):618-26.
Moroni A, Barbuti A, Altomare C, Viscomi C, Morgan J, Baruscotti M, DiFrancesco D.

1999

Action of internal pronase on the f-channel kinetics in the rabbit SA node.

J Physiol. 1999 Nov 1;520 Pt 3:737-44.
Barbuti A, Baruscotti M, Altomare C, Moroni A, DiFrancesco D.

The human gene coding for HCN2, a pacemaker channel of the heart.

Biochim Biophys Acta. 1999 Sep 3;1446(3):419-25.
Vaccari T, Moroni A, Rocchi M, Gorza L, Bianchi ME, Beltrame M, DiFrancesco D.

Action of serotonin on the hyperpolarization-activated cation current (Ih) in rat CA1 hippocampal neurons.

Eur J Neurosci. 1999 Sep;11(9):3093-100.
Gasparini S, DiFrancesco D.

Action of serotonin on the hyperpolarization-activated cation current (Ih) in rat CA1 hippocampal neurons.

Eur J Neurosci. 1999 Sep;11(9):3093-100.
Gasparini S, DiFrancesco D.

Dual allosteric modulation of pacemaker (f) channels by cAMP and voltage in rabbit SA node.

J Physiol. 1999 Mar 1;515 ( Pt 2):367-76.
DiFrancesco D.

1998

Two distinct pathways of muscarinic current responses in rabbit sino-atrial node myocytes.

Pflugers Arch. 1998 Dec;437(1):164-7.
Accili EA, Redaelli G, DiFrancesco D.

1997

Activation of f-channels by cAMP analogues in macropatches from rabbit sino-atrial node myocytes.

J Physiol. 1997 Jun 15;501 ( Pt 3):565-71.
Bois P, Renaudon B, Baruscotti M, Lenfant J, DiFrancesco D.

Differential control of the hyperpolarization-activated current (i(f)) by cAMP gating and phosphatase inhibition in rabbit sino-atrial node myocytes.

J Physiol. 1997 May 1;500 ( Pt 3):643-51.
Accili EA, Redaelli G, DiFrancesco D.

Reduction of K+ uptake in glia prevents long-term depression maintenance and causes epileptiform activity.

J Neurosci. 1997 Apr 15;17(8):2813-24.
Janigro D, Gasparini S, D'Ambrosio R, McKhann G 2nd, DiFrancesco D.

Properties and modulation of If in newborn versus adult cardiac SA node.

Am J Physiol. 1997 Mar;272(3 Pt 2):H1549-52.
Accili EA, Robinson RB, DiFrancesco D.

The newborn rabbit sino-atrial node expresses a neuronal type I-like Na+ channel.

J Physiol. 1997 Feb 1;498 ( Pt 3):641-8.
Baruscotti M, Westenbroek R, Catterall WA, DiFrancesco D, Robinson RB.

1996

Modulation of the hyperpolarization-activated current (I(f)) by adenosine in rabbit sinoatrial myocytes.

Circulation. 1996 Aug 15;94(4):734-41.
Zaza A, Rocchetti M, DiFrancesco D.

A TTX-sensitive inward sodium current contributes to spontaneous activity in newborn rabbit sino-atrial node cells.

J Physiol. 1996 Apr 1;492 ( Pt 1):21-30.
Baruscotti M, DiFrancesco D, Robinson RB.

Basal responses of the L-type Ca2+ and hyperpolarization-activated currents to autonomic agonists in the rabbit sino-atrial node.

J Physiol. 1996 Mar 1;491 ( Pt 2):347-55.
Zaza A, Robinson RB, DiFrancesco D.

Inhibition of the hyperpolarization-activated current (if) of rabbit SA node myocytes by niflumic acid.

Pflugers Arch. 1996 Mar;431(5):757-62.
Accili EA, DiFrancesco D.

Activation of the hyperpolarization-activated current (if) in sino-atrial node myocytes of the rabbit by vasoactive intestinal peptide.

Pflugers Arch. 1996 Mar;431(5):803-5.
Accili EA, Redaelli G, DiFrancesco D

Cytoskeletal control of rectification and expression of four substates in cardiac inward rectifier K+ channels.

FASEB J. 1996 Feb;10(2):357-61.
Mazzanti M, Assandri R, Ferroni A, DiFrancesco D

1995

Cesium and the pacemaker current.

J Cardiovasc Electrophysiol. 1995 Dec;6(12):1152-5.
DiFrancesco D.

The pacemaker current (I(f)) plays an important role in regulating SA node pacemaker activity.

Cardiovasc Res. 1995 Aug;30(2):307-8.
DiFrancesco D.

1994

Cesium prevents maintenance of long-term depression in rat hippocampal CA1 neurons.

Neuroreport. 1994 Sep 8;5(14):1813-6.
Maccaferri G, Janigro D, Lazzari A, DiFrancesco D.

Some properties of the UL-FS 49 block of the hyperpolarization-activated current (i(f)) in sino-atrial node myocytes.

Pflugers Arch. 1994 May;427(1-2):64-70.
DiFrancesco D.

Modulation of single hyperpolarization-activated channels (i(f)) by cAMP in the rabbit sino-atrial node.

J Physiol. 1994 Feb 1;474(3):473-82.
DiFrancesco D, Mangoni M.

1993

Properties of the hyperpolarization-activated current in rat hippocampal CA1 pyramidal cells.

J Neurophysiol. 1993 Jun;69(6):2129-36.
Maccaferri G, Mangoni M, Lazzari A, DiFrancesco D.

An isoform of the cGMP-gated retinal photoreceptor channel gene expressed in the sinoatrial node (pacemaker) region of rabbit heart.

Biochem Soc Trans. 1993 May;21(2):119S.
Hundal SP, DiFrancesco D, Mangoni M, Brammar WJ, Conley EC.

1992

Reciprocal role of the inward currents ib, Na and i(f) in controlling and stabilizing pacemaker frequency of rabbit sino-atrial node cells.

Proc Biol Sci. 1992 Dec 22;250(1329):199-207.
Noble D1, Denyer JC, Brown HF, DiFrancesco D.

Expression and characterization of a canine hippocampal inwardly rectifying K+ current in Xenopus oocytes.

J Physiol. 1992 Nov;457:229-46.
Cui J, Mandel G, DiFrancesco D, Kline RP, Pennefather P, Datyner NB, Haspel HC, Cohen IS.

Differential effects of ACh on cardiac pacemaker cells.

Trends Neurosci. 1992 Jul;15(7):249-50
DiFrancesco D, Ducouret P, Robinson R.

1991

Intracellular calcium does not directly modulate cardiac pacemaker (if) channels.

Pflugers Arch. 1991 Dec;419(6):662-4.
Zaza A1, Maccaferri G, Mangoni M, DiFrancesco D.

Effects of protein kinase inhibitors on canine Purkinje fibre pacemaker depolarization and the pacemaker current i(f).

J Physiol. 1991;440:367-84.
Chang F, Cohen IS, DiFrancesco D, Rosen MR, Tromba C.

The effects of manganese and barium on the cardiac pacemaker current, if, in rabbit sino-atrial node myocytes.

Experientia. 1991 May 15;47(5):449-52.
DiFrancesco D, Porciatti F, Cohen IS.

Direct activation of cardiac pacemaker channels by intracellular cyclic AMP.

Nature. 1991 May 9;351(6322):145-7.
DiFrancesco D1, Tortora P.

The contribution of the ‘pacemaker’ current (if) to generation of spontaneous activity in rabbit sino-atrial node myocytes.

J Physiol. 1991 Mar;434:23-40.
DiFrancesco D.

Block of the cardiac pacemaker current (If) in the rabbit sino-atrial node and in canine Purkinje fibres by 9-amino-1,2,3,4-tetrahydroacridine.

Pflugers Arch. 1991 Feb;417(6):611-5.
DiFrancesco D, Porciatti F, Janigro D, Maccaferri G, Mangoni M, Tritella T, Chang F, Cohen IS.

1990

Sodium current block caused by group IIb cations in calf Purkinje fibres and in guinea-pig ventricular myocytes.

Pflugers Arch. 1990 Oct;417(2):213-22.
Visentin S, Zaza A, Ferroni A, Tromba C, DiFrancesco C.

Acetylcholine reverses effects of beta-agonists on pacemaker current in canine cardiac Purkinje fibers but has no direct action. A difference between primary and secondary pacemakers.

Circ Res. 1990 Mar;66(3):633-6.
Chang F, Gao J, Tromba C, Cohen I, DiFrancesco D.

1989

Muscarinic modulation of cardiac rate at low acetylcholine concentrations.

Science. 1989 Feb 3;243(4891):669-71.
DiFrancesco D, Ducouret P, Robinson RB.

Intracellular Ca modulates K-inward rectification in cardiac myocytes.

Pflugers Arch. 1989 Jan;413(3):322-4.
Mazzanti M, DiFrancesco D.

Internal and external K+ help gate the inward rectifier.

Biophys J. 1989 Jan;55(1):197-202.
Cohen IS, DiFrancesco D, Mulrine NK, Pennefather P.

1988

Inhibition of the hyperpolarization-activated current (if) induced by acetylcholine in rabbit sino-atrial node myocytes.

J Physiol. 1988 Nov;405:477-91.
DiFrancesco D1, Tromba C.

Muscarinic control of the hyperpolarization-activated current (if) in rabbit sino-atrial node myocytes.

J Physiol. 1988 Nov;405:493-510.
DiFrancesco D1, Tromba C.

1987

Acetylcholine inhibits activation of the cardiac hyperpolarizing-activated current, if.

Pflugers Arch. 1987 Sep;410(1-2):139-42.
DiFrancesco D, Tromba C.

1986

Characterization of single pacemaker channels in cardiac sino-atrial node cells.

Nature. 1986 Dec 4-10;324(6096):470-3.
DiFrancesco D.

Properties of the hyperpolarizing-activated current (if) in cells isolated from the rabbit sino-atrial node.

J Physiol. 1986 Aug;377:61-88.
DiFrancesco D, Ferroni A, Mazzanti M, Tromba C.

1985

Cadmium-induced blockade of the cardiac fast Na channels in calf Purkinje fibres.

Proc R Soc Lond B Biol Sci. 1985 Feb 22;223(1233):475-84.
DiFrancesco D, Ferroni A, Visentin S, Zaza A.

A model of cardiac electrical activity incorporating ionic pumps and concentration changes.

Philos Trans R Soc Lond B Biol Sci. 1985 Jan 10;307(1133):353-98.
DiFrancesco D, Noble D.

1984

Characterization of the pacemaker (if) current kinetics in calf Purkinje fibres.

J.Physiol. 348, 341-367
DiFrancesco,D. (1984)

Barium-induced blockade of the inward rectifier in calf Purkinje fibres.

Pflügers Arch. 402, 446-453
DiFrancesco,D. & Ferroni,A. & Visentin,S. (1984)

1983

Delayed activation of the cardiac pacemaker current and its dependence on conditioning pre- hyperpolarization.

Pflügers Arch. 396, 265-276
DiFrancesco,D. & Ferroni,A. (1983)

1982

Block and activation of the pacemaker if channel in calf Purkinje fibres: effects of potassium, caesium and rubidium.

J.Physiol. 329, 485-507
DiFrancesco,D. (1982)

1981

A new interpretation of the pacemaker current iK2 in calf Purkinje fibres.

J.Physiol. 314, 359-376
DiFrancesco,D. (1981a)

A study of the ionic nature of the pacemaker current in calf Purkinje fibres.

J.Physiol. 314, 377-393
DiFrancesco,D. (1981b)

1980

Separation of current induced by potassium accumulation from acetylcholine-induced relaxation current in the rabbit SA node.

Pflügers Arch. 387, 83-90
DiFrancesco,D., Noma,A. & Trautwein,W. (1980)

The contribution of potassium accumulation to outward currents in frog atrium.

J.Physiol. 306, 127-149
Brown,H.F., DiFrancesco,D., Noble,D. & Noble,S.J. (1980)

The time course of potassium current following potassium accumulation: analytical solution using a linear approximation.

J.Physiol. 306, 151-173
DiFrancesco,D. & Noble,D. (1980)

Voltage-clamp investigations of membrane currents underlying pacemaker activity in rabbit sino- atrial node.

J.Physiol. 308, 331-351
Brown,H.F. & DiFrancesco,D. (1980)

Properties of the current if in the sinoatrial node of the rabbit compared with those of the current iK2 in Purkinje fibres.

J.Physiol. 308, 353-367
DiFrancesco,D. & Ojeda,C. (1980)

1979

The effects of Calcium on outward membrane currents in the cardiac Purkinje fibres

J Physiol. 1979; 289, 347-373
DiFrancesco,D. & McNaughton,P.A.

How does adrenaline accelerate the heart?

Nature, 280, 235-236
Brown,H.F., DiFrancesco,D. & Noble,S.J. (1979a)

Measurement and significance of the reversal potential for the pacemaker current iK2 in Purkinje fibres.

J.Physiol. 297, 135-162
DiFrancesco,D., Ohba,M. & Ojeda,C. (1979)

Cardiac pacemaker oscillation and its modulation by autonomic transmitters.

J.Exp.Biol. 81, 175-204
Brown,H.F., DiFrancesco,D. & Noble,S.J. (1979b)

Kinetics and magnitude of the time-dependent potassium current in the rabbit sino-atrial node: effect of external potassium.

Pflügers Arch. 381, 271-279
DiFrancesco,D., Noma,A. & Trautwein,W. (1979)

1978

Dependence of the apparent reversal potential for the pace-maker current iK2 on its degree of activation in cardiac Purkinje fibres

J Physiol. 1978 Jul;280:73P-74P.
DiFrancesco D, Ohba M.